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Jezewski, Andrew J; Esan, Taiwo E; Propp, Jonah; Fuller, Andrew J; Daraji, Drashti G; Lail, Charles; Staker, Bart L; Woodward, Elijah L; Liu, Linjun; Battaile, Kevin P; et al (, Journal of Biological Chemistry)
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DeBouver, Nicholas D.; Bolejack, Madison J.; Esan, Taiwo E.; Krysan, Damian J.; Hagen, Timothy J.; Abendroth, Jan (, Acta Crystallographica Section F Structural Biology Communications)The compound ethyl-adenosyl monophosphate ester (ethyl-AMP) has been shown to effectively inhibit acetyl-CoA synthetase (ACS) enzymes and to facilitate the crystallization of fungal ACS enzymes in various contexts. In this study, the addition of ethyl-AMP to a bacterial ACS from Legionella pneumophila resulted in the determination of a co-crystal structure of this previously elusive structural genomics target. The dual functionality of ethyl-AMP in both inhibiting ACS enzymes and promoting crystallization establishes its significance as a valuable resource for advancing structural investigations of this class of proteins.more » « less
